Coding

Part:BBa_K1732019

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-16)

E0040-HIS

GFP with 6X His Tag for easier protein purification. E0040 (BBa_E0040) was the GFP sequence used in the iGEM 2015 interlab study.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


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Categories
Parameters
None